{"id":10545,"date":"2010-02-05T11:24:30","date_gmt":"2010-02-05T11:24:30","guid":{"rendered":"http:\/\/www.eurekaone.com\/s6-en-articoli\/c42-en-kits-in-hplc\/25-esandione-urinario-totale-in-uv-2\/"},"modified":"2025-06-18T12:25:27","modified_gmt":"2025-06-18T10:25:27","slug":"25-esandione-urinario-totale-in-uv-2","status":"publish","type":"post","link":"https:\/\/www.eurekakit.com\/en\/25-esandione-urinario-totale-in-uv-2\/","title":{"rendered":"Total 2,5 Hexanedione in urine by UV – FAST"},"content":{"rendered":"\n\n\n
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Total 2,5 Hexanedione in urine by UV – FAST (code\u00a0 Z05210<\/span><\/strong>) – 100 tests package<\/p>\n

INSIGHTS<\/strong>
\n2,5 Hexanedione is an aliphatic diketone. It is a product of biochemical transformation of hexane. Hexane is a common component of glues used in the production of shoes or leathers. Inhalation is the major way of absorption, while ingestion, eye and skin absorption can also occur. It is then metabolised into 2,5 hexanedione and eliminated by urine. For these reasons 2,5 hexanedione testing is used to determine the risk of exposure to hexane. Additionally, 2,5 hexanedione levels are related to the quantity of hexane adsorbed. From a clinical point of view 2,5 hexanedione is considered one of the most important metabolites of hexane in the development of polyneuropathies in leather workers and shoemakers.<\/p>\n

Principle of the Method:<\/span><\/strong><\/p>\n

2,5 Hexanedione-glucuronide is hydrolyzed in acid ambient at 100 \u00b0C for 1 hour. After cooling, the derivatization reagent is added and the solution is incubated at 70 \u00b0C for 30 minutes. After cooling again, 50 \u03bcl of solution are directly injected into the HPLC.<\/p>\n\n\n\n\n
\"right_blue_arrow\"<\/a><\/td>\nIFU<\/span><\/strong><\/a><\/td>\n<\/tr>\n
\"right_blue_arrow\"<\/td>\nIFU MOBILE PHASE<\/span><\/strong><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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 <\/p>\n","protected":false},"excerpt":{"rendered":"

Total 2,5 Hexanedione in urine by UV – FAST (code\u00a0 Z05210) – 100 tests package INSIGHTS 2,5 Hexanedione is an aliphatic diketone. 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