{"id":8309,"date":"2016-05-24T13:57:07","date_gmt":"2016-05-24T13:57:07","guid":{"rendered":"http:\/\/www.eurekaone.com\/senza-categoria\/cortisol-and-cortisone-in-urine-by-lcms\/"},"modified":"2025-06-10T11:09:58","modified_gmt":"2025-06-10T09:09:58","slug":"cortisol-and-cortisone-in-urine-by-lcms","status":"publish","type":"post","link":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/","title":{"rendered":"Cortisol and Cortisone in urine by LC\/MS 100 test"},"content":{"rendered":"\n\n\n
\"Prodotti\"<\/td>\n<\/td>\n\n

Cortisol and Cortisone in urine by LC\/MS (code\u00a0 LC91010<\/span><\/strong>)\u00a0 100 test package.<\/p>\n

INSIGHTS<\/strong>
\nCortisol is a steroid hormone synthetized in the adrenal gland, starting from cholesterol, through an enzymatic cascade. It is the principal glucocorticoid in humans and it acts as a gene transcription factor influencing a variety of cellular responses in many tissues. Cortisol plays a key role in the metabolism of glucose, in the maintenance of vascular tone as well as regulates the immune response and the body\u2019s response to stress. Only a small percentage of circulating cortisol is biologically active, most cortisol is inactive (protein bound). When the plasma cortisol value rises, the free cortisol also increases, and is filtered by the glomerulus. The urinary free cortisol is well correlated with the concentration of serum free cortisol. The urinary free cortisol is biologically active, and is responsible for the signs and symptoms of hypercortisolism (Cushing\u2019s Syndrome). The measurement of free cortisol excretion over 24 hours associated with liquid mass spectrometry (LCMS\/ MS) is the preferred screening test for Cushing\u2019s syndrome. Cortisone, cortisol metabolite, is an additional parameter to help in the diagnosis of various adrenal disorders, including the abnormality in the activity of 11-beta-hydroxy-dehydrogenase (11-beta HSD), the enzyme that converts cortisol to cortisone. This method allows to determine simultaneously both the cortisol and Cortisone in 24-hour urine sample by LCMS\/ MS.<\/p>\n

Principle of the\u00a0 Method:<\/span><\/strong><\/p>\n

Urine is diluted with appropriate reagent containing the internal standard and injected into the LC-MS\/MS system.<\/p>\n\n\n\n\n\n\n
\"right_blue_arrow\"<\/a><\/td>\nIFU<\/span><\/strong><\/a><\/td>\n<\/tr>\n
\"right_blue_arrow\"<\/a><\/td>\nIFU MOBILE PHASE\u00a0 M1\u00a0<\/span><\/strong><\/a><\/td>\n<\/tr>\n
\"right_blue_arrow\"<\/a><\/td>\nIFU MOBILE PHASE\u00a0 M2<\/span><\/strong><\/a><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
\"Torna<\/a><\/td>\n<\/td>\nback to previous page<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Cortisol and Cortisone in urine by LC\/MS (code\u00a0 LC91010)\u00a0 100 test package. INSIGHTS Cortisol is a steroid hormone synthetized in the adrenal gland, starting from cholesterol, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[422],"tags":[],"class_list":["post-8309","post","type-post","status-publish","format-standard","hentry","category-recent-products"],"yoast_head":"\nCortisol and Cortisone in urine by LC\/MS 100 test - Eureka Kit<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cortisol and Cortisone in urine by LC\/MS 100 test - Eureka Kit\" \/>\n<meta property=\"og:description\" content=\"Cortisol and Cortisone in urine by LC\/MS (code\u00a0 LC91010)\u00a0 100 test package. INSIGHTS Cortisol is a steroid hormone synthetized in the adrenal gland, starting from cholesterol, […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/\" \/>\n<meta property=\"og:site_name\" content=\"Eureka Kit\" \/>\n<meta property=\"article:published_time\" content=\"2016-05-24T13:57:07+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-06-10T09:09:58+00:00\" \/>\n<meta name=\"author\" content=\"ANg1a_cUnza\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"ANg1a_cUnza\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/\",\"url\":\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/\",\"name\":\"Cortisol and Cortisone in urine by LC\/MS 100 test - Eureka Kit\",\"isPartOf\":{\"@id\":\"https:\/\/www.eurekakit.com\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#primaryimage\"},\"thumbnailUrl\":\"http:\/\/www.eurekakit.com\/wp-content\/uploads\/2006\/10\/prodotti.gif\",\"datePublished\":\"2016-05-24T13:57:07+00:00\",\"dateModified\":\"2025-06-10T09:09:58+00:00\",\"author\":{\"@id\":\"https:\/\/www.eurekakit.com\/#\/schema\/person\/add3353ed09549865e7afe5ae38368ec\"},\"breadcrumb\":{\"@id\":\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#primaryimage\",\"url\":\"http:\/\/www.eurekakit.com\/wp-content\/uploads\/2006\/10\/prodotti.gif\",\"contentUrl\":\"http:\/\/www.eurekakit.com\/wp-content\/uploads\/2006\/10\/prodotti.gif\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.eurekakit.com\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Cortisol and Cortisone in urine by LC\/MS 100 test\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.eurekakit.com\/#website\",\"url\":\"https:\/\/www.eurekakit.com\/\",\"name\":\"Eureka Kit\",\"description\":\"Eurekakit la pi\u00f9 Ampia Gamma al Mondo di Kit Diagnostici per Strumenti Analitici Cromatografici HPLC, GC, GC-MS e LC-MS\/MS,\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.eurekakit.com\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.eurekakit.com\/#\/schema\/person\/add3353ed09549865e7afe5ae38368ec\",\"name\":\"ANg1a_cUnza\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.eurekakit.com\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/1f76f07af7ba5f52944f97ed8a7ffa64?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/1f76f07af7ba5f52944f97ed8a7ffa64?s=96&d=mm&r=g\",\"caption\":\"ANg1a_cUnza\"},\"url\":\"https:\/\/www.eurekakit.com\/en\/author\/ang1a_cunza\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Cortisol and Cortisone in urine by LC\/MS 100 test - Eureka Kit","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/","og_locale":"en_US","og_type":"article","og_title":"Cortisol and Cortisone in urine by LC\/MS 100 test - Eureka Kit","og_description":"Cortisol and Cortisone in urine by LC\/MS (code\u00a0 LC91010)\u00a0 100 test package. INSIGHTS Cortisol is a steroid hormone synthetized in the adrenal gland, starting from cholesterol, […]","og_url":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/","og_site_name":"Eureka Kit","article_published_time":"2016-05-24T13:57:07+00:00","article_modified_time":"2025-06-10T09:09:58+00:00","author":"ANg1a_cUnza","twitter_card":"summary_large_image","twitter_misc":{"Written by":"ANg1a_cUnza","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/","url":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/","name":"Cortisol and Cortisone in urine by LC\/MS 100 test - Eureka Kit","isPartOf":{"@id":"https:\/\/www.eurekakit.com\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#primaryimage"},"image":{"@id":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#primaryimage"},"thumbnailUrl":"http:\/\/www.eurekakit.com\/wp-content\/uploads\/2006\/10\/prodotti.gif","datePublished":"2016-05-24T13:57:07+00:00","dateModified":"2025-06-10T09:09:58+00:00","author":{"@id":"https:\/\/www.eurekakit.com\/#\/schema\/person\/add3353ed09549865e7afe5ae38368ec"},"breadcrumb":{"@id":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#primaryimage","url":"http:\/\/www.eurekakit.com\/wp-content\/uploads\/2006\/10\/prodotti.gif","contentUrl":"http:\/\/www.eurekakit.com\/wp-content\/uploads\/2006\/10\/prodotti.gif"},{"@type":"BreadcrumbList","@id":"https:\/\/www.eurekakit.com\/en\/cortisol-and-cortisone-in-urine-by-lcms\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.eurekakit.com\/en\/"},{"@type":"ListItem","position":2,"name":"Cortisol and Cortisone in urine by LC\/MS 100 test"}]},{"@type":"WebSite","@id":"https:\/\/www.eurekakit.com\/#website","url":"https:\/\/www.eurekakit.com\/","name":"Eureka Kit","description":"Eurekakit la pi\u00f9 Ampia Gamma al Mondo di Kit Diagnostici per Strumenti Analitici Cromatografici HPLC, GC, GC-MS e LC-MS\/MS,","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.eurekakit.com\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/www.eurekakit.com\/#\/schema\/person\/add3353ed09549865e7afe5ae38368ec","name":"ANg1a_cUnza","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.eurekakit.com\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/1f76f07af7ba5f52944f97ed8a7ffa64?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/1f76f07af7ba5f52944f97ed8a7ffa64?s=96&d=mm&r=g","caption":"ANg1a_cUnza"},"url":"https:\/\/www.eurekakit.com\/en\/author\/ang1a_cunza\/"}]}},"_links":{"self":[{"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/posts\/8309","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/comments?post=8309"}],"version-history":[{"count":14,"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/posts\/8309\/revisions"}],"predecessor-version":[{"id":26146,"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/posts\/8309\/revisions\/26146"}],"wp:attachment":[{"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/media?parent=8309"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/categories?post=8309"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.eurekakit.com\/en\/wp-json\/wp\/v2\/tags?post=8309"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}