{"id":9802,"date":"2016-05-24T13:57:07","date_gmt":"2016-05-24T13:57:07","guid":{"rendered":"http:\/\/www.eurekaone.com\/senza-categoria\/tamoxifen-endoxifen-in-plasma-by-lcms\/"},"modified":"2025-06-16T10:01:06","modified_gmt":"2025-06-16T08:01:06","slug":"5-fluorouracil-in-pbmc-by-lc-msms","status":"publish","type":"post","link":"https:\/\/www.eurekakit.com\/en\/5-fluorouracil-in-pbmc-by-lc-msms\/","title":{"rendered":"5-Fluorouracil in PBMC BY LC-MS\/MS"},"content":{"rendered":"\n\n\n
\"Prodotti\"<\/td>\n<\/td>\n\n

5-Fluorouracil in PBMC BY LC-MS\/MS \u00a0(code\u00a0 LC85010<\/span><\/strong>)\u00a0 100 test package.<\/p>\n

INSIGHTS<\/strong>
\nFluorouracil (5-FU) is considered the most efficient and most used antineoplastic agent for the treatment of gastrointestinal, breast and ovary tumors. 5-FU acts by antagonizing thymidylate synthase (TS). Its conversion to 5 -Fluoro – 2\u2019deossi – 5\u2019-monophosphate (FdUMP) leads to inhibition of TS thus blocking the synthesis of DNA and interrupting the whole transcriptional process and protein synthesis. The great efficacy of this drug, however, goes hand in hand with its low therapeutic index. 5-FU is catabolized primarily by the enzyme dihydropyrimidine dehydrogenase and patients with complete or partial functional deficiency of this enzyme are likely to develop side effects such as cardiac toxicity, myelosuppression and renal failure. This method allows to determine, exvivo, the rate of degradation of the drug 5-fluorouracil (5-FU). After purification of peripheral blood lymphocytes, the cells are incubated with a known amount of 5-FU, and the amount of drug metabolised after 2 hours is determined by LC-MS\/MS. The present method allows the evaluation in a preventive manner of the rate of degradation of 5 – fluorouracil, in order to highlight the toxic risk to an individual before administration of the therapy<\/p>\n

Principle of the\u00a0 Method:<\/span><\/strong><\/p>\n

This method allows to determine, ex-vivo, the rate of degradation of the drug 5-fluorouracil (5-FU). After purification of peripheral blood lymphocytes, the cells are incubated with a known amount of 5-FU, and the amount of drug metabolised after 2 hours is determined by LC-MS\/MS .<\/p>\n\n\n\n\n\n
\"right_blue_arrow\"<\/a><\/td>\nIFU<\/span><\/strong><\/a><\/td>\n<\/tr>\n
\"right_blue_arrow\"<\/a><\/td>\nIFU MOBILE\u00a0<\/span><\/strong>PHASE M1<\/span><\/strong><\/a><\/td>\n<\/tr>\n
\"right_blue_arrow\"<\/a><\/td>\nIFU MOBILE\u00a0<\/span><\/strong>PHASE M2<\/span><\/strong><\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\"Torna<\/td>\n<\/td>\nback to previous page<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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5-Fluorouracil in PBMC BY LC-MS\/MS \u00a0(code\u00a0 LC85010)\u00a0 100 test package. INSIGHTS Fluorouracil (5-FU) is considered the most efficient and most used antineoplastic agent for the treatment […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[422],"tags":[],"class_list":["post-9802","post","type-post","status-publish","format-standard","hentry","category-recent-products"],"yoast_head":"\n5-Fluorouracil in PBMC BY LC-MS\/MS - Eureka Kit<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.eurekakit.com\/en\/5-fluorouracil-in-pbmc-by-lc-msms\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"5-Fluorouracil in PBMC BY LC-MS\/MS - Eureka Kit\" \/>\n<meta property=\"og:description\" content=\"5-Fluorouracil in PBMC BY LC-MS\/MS \u00a0(code\u00a0 LC85010)\u00a0 100 test package. 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