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Free 2,5 Hexanedione in urine by Fluorimetry – FAST (code Z05910) – 100 tests package. INSIGHTS 2,5 Hexanedione is an aliphatic diketone. It is a product of biochemical transformation of hexane. Hexane is acommon component of glues used in the production of shoes or leathers. Inhalation is the major way of absorption, while ingestion, eye and skin absorption can also occur. It is then metabolised into 2,5 hexanedione and eliminated by urine. For these reasons 2,5 hexanedione testing is used to determine the risk of exposure to hexane. Additionally, 2,5 hexanedione levels are related to the quantity of hexane adsorbed. From a clinical point of view 2,5 hexanedione is considered one of the most important metabolites of hexane in the development of polyneuropathies in leather workers and shoemakers Principle of the Method: 2,5 Hexanedione is derivatized with a specific reagent and, after purification in a clean up column, it is injected into the HPLC System.
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