The activity in this report was carried out at the Forensic Toxicology Chemistry Laboratory of the Institute of Medical Sciences of the University of Macerata. This subject of this technical-scientific activity was the comparison of two different extractive procedures for SPE. In particular, methods used in analytical activity were compared with those developed by Eureka-Lab Division. Techniques for Urinary Opioid and Cannabinoid Analysis (Delta 9 THC-COOH) were evaluated. The first phase of the activity concerned the comparison of the two methods using calibrators of the two known concentrations, supplied by Eureka. Subsequently, the comparison covered urinary samples at the Forensic Toxic Chemistry Laboratory, selected on the basis of the positivity for the above mentioned substances. The sample was divided into two equal volumes. An aliquot was extracted with the lab method, while the other was subjected to extraction using the Eureka kit supplied and according to the procedures of the company.
The extracts were then analyzed by gas-chromatography-mass spectrometry (GCIMS).
The results of the partial procedure can be summarized in:
1) The methods developed by Eureka are not very expensive in terms of time and are practical for the use of ready solutions.
2) Compared to the methods used in the laboratory, the methods produce very clean extracts that have improved the quantizational response of the GC / MS analytics.
Ultimately, the proposed, quick and practical procedures have a particular efficacy in the purification phase of the extracts.