Hydroxyproline in urine by UV / VIS FAST

INSIGHTS

Hydroxyproline is contained almost exclusively in collagen, where it accounts for 14% of the total aminoacid-composition and comes from hydroxylation of proline. Collagen, the principal component of bones, is a protein whose primary structure is made by a repetitive sequence of glycine, proline, hydroxyproline and hydroxylysine. Since half of the total collagen is in the bone matter, a bone reabsorption causes an impoverishment in collagen and presents itself with a higher concentration of hydroxylysine proportional to the amount of destructed collagen. Urinary hydroxyproline is an important marker of bone resorption. Its urinary excretion is an index of the rate of bone resorption.

Hydroxyproline is derivatized and injected into the HPLC after acid hydrolysis at 100 °C for 16 h.

	IFU
	IFU MOBILE PHASE