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1-Hydroxypyrene in urine by Fluorimetry FAST (code Z38210) 100 test package. INSIGHTS 1-Hydroxypyrene is a metabolic product of polycyclic aromatic hydrocarbons (PAH) in human urine. The generic term PAH represents more than 200 aromatic compounds with condensed ring systems. Just as a large number of PAHs have been shown to be highly carcinogenic in animal experiments, exposure to PAH can be a serious danger to human organism as well. Intake of PAHs may occur primarily in the lungs and the gastro-intestinal tract or through the skin as a secondary route. 1 – Hydroxypyrene is excreted in urine conjugated with glucuronic acid and with sulfate. To free it for analysis, the sample is hydrolyzed with β-glucuronidase arilsulfatase for 16 h at 37 °C at pH=5.0. Among PAH metabolites 1-hydroxypyrene is the most important biomarker for the evaluation of occupational related PAH exposure Principle of the Method: 1-HYDROXYPYRENE, after enzymatic hydrolysis at pH=5,0 and after purification in a clean up column it is injected into the HPLC System.
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