Total 2,5 Hexanedione in urine by UV – FAST

Total 2,5 Hexanedione in urine by UV – FAST (code  Z05210) – 100 tests package

INSIGHTS
2,5 Hexanedione is an aliphatic diketone. It is a product of biochemical transformation of hexane. Hexane is a common component of glues used in the production of shoes or leathers. Inhalation is the major way of absorption, while ingestion, eye and skin absorption can also occur. It is then metabolised into 2,5 hexanedione and eliminated by urine. For these reasons 2,5 hexanedione testing is used to determine the risk of exposure to hexane. Additionally, 2,5 hexanedione levels are related to the quantity of hexane adsorbed. From a clinical point of view 2,5 hexanedione is considered one of the most important metabolites of hexane in the development of polyneuropathies in leather workers and shoemakers.

Principle of the Method:

2,5 Hexanedione-glucuronide is hydrolyzed in acid ambient at 100 °C for 1 hour. After cooling, the derivatization reagent is added and the solution is incubated at 70 °C for 30 minutes. After cooling again, 50 μl of solution are directly injected into the HPLC.

	IFU
	IFU MOBILE PHASE