Cortisol and Cortisone in urine by LC/MS 100 test

Cortisol and Cortisone in urine by LC/MS (code  LC91010)  100 test package.

INSIGHTS
Cortisol is a steroid hormone synthetized in the adrenal gland, starting from cholesterol, through an enzymatic cascade. It is the principal glucocorticoid in humans and it acts as a gene transcription factor influencing a variety of cellular responses in many tissues. Cortisol plays a key role in the metabolism of glucose, in the maintenance of vascular tone as well as regulates the immune response and the body’s response to stress. Only a small percentage of circulating cortisol is biologically active, most cortisol is inactive (protein bound). When the plasma cortisol value rises, the free cortisol also increases, and is filtered by the glomerulus. The urinary free cortisol is well correlated with the concentration of serum free cortisol. The urinary free cortisol is biologically active, and is responsible for the signs and symptoms of hypercortisolism (Cushing’s Syndrome). The measurement of free cortisol excretion over 24 hours associated with liquid mass spectrometry (LCMS/ MS) is the preferred screening test for Cushing’s syndrome. Cortisone, cortisol metabolite, is an additional parameter to help in the diagnosis of various adrenal disorders, including the abnormality in the activity of 11-beta-hydroxy-dehydrogenase (11-beta HSD), the enzyme that converts cortisol to cortisone. This method allows to determine simultaneously both the cortisol and Cortisone in 24-hour urine sample by LCMS/ MS.

Principle of the  Method:

Urine is diluted with appropriate reagent containing the internal standard and injected into the LC-MS/MS system.

	IFU
	IFU MOBILE PHASE  M1
	IFU MOBILE PHASE  M2