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Opiates in urine by detector MS (Morphine, Codeine, Dihydrocodeine, Ethylmorphine, 6-Monoacethylmorphine) – (code GC45010) – 50 tests package.
INSIGHTS
Heroin (Diacetylmorphine) is a semisynthetic substance of abuse obtained from Morphine, one of the principal opioid alkaloids, by reaction with acetic anhydride. Inside the organism, Heroin is rapidly hydrolyzed into 6-Monoacetylmorphine and then Morphine. The latter is then conjugated with Glucuronic Acid in positions 3 and 6, forming morphine-3-glucuronide and morphine-6-glucuronide. The last two are expelled in urine together with a small quantity of free Morphine. In opium,it is also possible to find small quantities of Codeine,and Acetylcodeine, obtained by acetylation of Codeine during the preparation of Heroin. The methods of 1° screening for the determination of opiates, through a reaction antigen-antibody, require a confirmatory analysis by chromatographic technique.This method by GC-MS allows to determine the total urinary morphine (after hydrolysis of the glucuronides), codeine, 6-monoacetylmorphine. Moreover, it also determines dihydrocodeine and ethylmorphine which are components of some pharmaceutical preparations. This is a remarkable feature because it allows to differentiate them from the other molecules contained in opium.
Principle of the Method:
This method allows to determinate the Morphine in urine (the main metabolite of Heroin), the Dihydrocodeine, the Codeine, the Ethylmorphine and the 6-Monoacethylmorphine after hydrolysis of urines and SPE extraction and reading in GC-MS..
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