Free Eritrocitary Protoporphyrin IX and Zinc Protoporphyrin (ZnPP) in whole blood by Fluorimetry – FAST

Free Eritrocitary Protoporphyrin IX and ZnPP in whole blood by Fluorimetry – FAST (code  Z39110)  – 100 tests package.

INSIGHTS
Porphyrias are a group of hereditary disorders resulting from enzymatic defects in the biosynthetic pathway of heme. Depending on the specific enzyme involved, various porphyrins and their precursors accumulate in different types of samples. Eritrocitary Protoporphyrin IX (PPIX) analysis should be performed in patients with clinical suspicion of erythropoietic protoporphyria (EPP) or X-linked dominant protoporphyria (XLDPP). Common symptoms may include itching, edema, erythema, burning or burning sensation, and occasionally scarring of the skin in sun-exposed areas.

Principle of the  Method:

This method determines the concentrations of PROTOPORPHYRIN IX and ZincPROTOPORPHYRIN in Whole Blood (WB). The Internal Standard (50 μl) and a Precipitation Reagent (600 μl) are added to 200 μl of WB; after 20 sec. at vortex and 5 minutes in microcentrifuge at 10.000 rpm, 20 μl are injected into HPLC system.

	IFU
	IFU MOBILE PHASE