up to 20 µg/l

92 %

CV = 0.9–2.7 %

CV = 2.7–5.7 %

For non-smokers aged 3 to 69 years, the following reference value applies:

1-Hydroxypyrene: 0.5 µg/l (0.3 µg/g creatinine)

Reference value, also known as Biological Reference Value (BRV) = 95^th percentile of frequency distribution of urinary 1-hydroxypyrene concentration in the non-occupationally exposed population

Urine

Patient specimens can be kept for up to 2 days at room temperature and at +2 to +8 °C. For longer periods of storage (up to 1 year), deep-freeze below –18 °C.

ENZYMATIC HYDROLYSIS

1. Mix (vortex for 2 s) 1 ml urine (sample/control/calibrator) with 50 µl internal standard and 200 µl of the prepared enzyme solution in a reaction vial
2. Adjust pH if necessary (prepared enzyme solution includes colour indicator)
3. Close the vial tightly and incubate for at least 2 h at 37 °C

SOLID PHASE EXTRACTION

1. Briefly mix (vortex for 2 s) the hydrolysed urine
2. Add 1 ml of the hydrolysed urine to a Sample Clean Up Column and draw through by centrifugation (2 min at 700 x g) or suction. Discard effluent.
3. Centrifuge (1 min at 700 x g) 1000 µl Wash Buffer through the Sample Clean Up Column. Discard effluent.
4. Centrifuge (2 min at 700 x g) 300 µl Elution Buffer through the Sample Clean Up Column into a light-protected collection vessel (e.g. autosampler vial)
5. Briefly mix (vortex for 2 s) the eluate
6. Inject 10 µl eluate into the HPLC system

The prepared samples can be kept for up to two weeks at +18 to +30 °C, at +2 to +8 °C or below –18 °C.

For the HPLC analysis of 1-hydroxypyrene in urine any isocratic system with fluorescence detector is suitable.

10 µl

1.2 ml/min

35 °C

EX 242 nm
EM 388 nm

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manual