IDROSSIPROLINA URINARIA UV/VIS – FAST

        Cortisone           Cortisone
       Cortisone           Cortisone
       Cortisone           Cortisone
       Cortisone           Cortisone
       Cortisone           Cortisone
       Cortisone          Cortisone

Ordine n°Z01610                             Number of Test: 100

DESCRIPTION

L’Idrossiprolina urinaria in UV / VIS FAST (codice  Z01610) viene prodotta in confezioni da 100 test.

Principio del Metodo: 

L’idrossiprolina, dopo idrolisi acida a 110 °C per 16 ore, viene derivatizzata e iniettata nell’HPLC.

right_blue_arrow Metodologia d’uso

CHROMATOGRAM

53000 HPLC Kit 1-Hydroxypyrene in Urine -Chromatogram EN

TECHNICAL DATA

RECUPERO DEL METODO 100%
NUMBER OF TESTS 100
SENSIBILITÀ DEL METODO 0,1 mg/l
RANGE DINAMICO DEL METODO 0,1 – 300 mg/l
RANGE DI NORMALITÀ NELLE URINE DELLE 24 H 10,4 – 22,6 mg/l

Linearity

up to 20 µg/l

Recovery

92 %

Intraassay

CV = 0.9–2.7 %

Interassay

CV = 2.7–5.7 %

Reference ranges

For non-smokers aged 3 to 69 years, the following reference value applies:

1-Hydroxypyrene: 0.5 µg/l (0.3 µg/g creatinine)

Reference value, also known as Biological Reference Value (BRV) = 95th percentile of frequency distribution of urinary 1-hydroxypyrene concentration in the non-occupationally exposed population

Specimen

Urine

Pre-analytic Treatment

Patient specimens can be kept for up to 2 days at room temperature and at +2 to +8 °C. For longer periods of storage (up to 1 year), deep-freeze below –18 °C.

Sample Preparation

ENZYMATIC HYDROLYSIS

  1. Mix (vortex for 2 s) 1 ml urine (sample/control/calibrator) with 50 µl internal standard and 200 µl of the prepared enzyme solution in a reaction vial
  2. Adjust pH if necessary (prepared enzyme solution includes colour indicator)
  3. Close the vial tightly and incubate for at least 2 h at 37 °C

SOLID PHASE EXTRACTION

  1. Briefly mix (vortex for 2 s) the hydrolysed urine
  2. Add 1 ml of the hydrolysed urine to a Sample Clean Up Column and draw through by centrifugation (2 min at 700 x g) or suction. Discard effluent.
  3. Centrifuge (1 min at 700 x g) 1000 µl Wash Buffer through the Sample Clean Up Column. Discard effluent.
  4. Centrifuge (2 min at 700 x g) 300 µl Elution Buffer through the Sample Clean Up Column into a light-protected collection vessel (e.g. autosampler vial)
  5. Briefly mix (vortex for 2 s) the eluate
  6. Inject 10 µl eluate into the HPLC system

Sample Stability

The prepared samples can be kept for up to two weeks at +18 to +30 °C, at +2 to +8 °C or below –18 °C.

Additional Info

For the HPLC analysis of 1-hydroxypyrene in urine any isocratic system with fluorescence detector is suitable.

Injection Volume

10 µl

Flow rate

1.2 ml/min

Column temperature

35 °C

Wavelength

EX 242 nm
EM 388 nm

Please note

The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manual

KIT COMPONENTS

Fase Mobile

Order n°: 002152

Hidroxypene

Order n°: 002152

Details

Hidroxypene

Order n°: 002152

Details

Hidroxypene

Order n°: 002152

Details

Hidroxypene

Order n°: 002152

Details

Hidroxypene

Order n°: 002152

Details